Journal: Scientific Reports

Article Title: Genome-wide analysis suggests a differential microRNA signature associated with normal and diabetic human corneal limbus

doi: 10.1038/s41598-017-03449-7

Figure Lengend Snippet: List of antibodies used in this study.

Article Snippet: Ki-67 , Rabbit mAb ab92742 , Abcam , 359 , 1:200.

Techniques:

Journal: NPJ Breast Cancer

Article Title: Targeting GD2 with naxitamab overcomes GD3 synthase-driven immune suppression in triple-negative breast cancer

doi: 10.1038/s41523-025-00843-7

Figure Lengend Snippet: A Analysis of GD3S ( ST8SIA1 ) mRNA expression in METABRIC and TCGA (pan cancer) BC patient datasets shows significant correlation with immune checkpoint inhibitors. In the METABRIC dataset, Pearson’s correlation coefficients indicate significant correlations of GD3S mRNA expression with CTLA-4 ( r = 0.4; P < 0.001), LAG3 ( r = 0.33; P < 0.001), PD1 ( r = 0.3; P < 0.001), CD25 ( r = 0.38; P < 0.001), and SIRPα ( r = 0.36; P < 0.001). Similarly, in the TCGA dataset, GD3S mRNA expression was significantly correlated with CTLA-4 ( r = 0.47; P < 0.001), LAG3 ( r = 0.33; P < 0.001), PD1 ( r = 0.38; P < 0.001), CD25 ( r = 0.45; P < 0.001), and SIRPα ( r = 0.56; P < 0.001). B Representative IHC images obtained by sequential multiplex immunofluorescence images (Lunaphore COMET) from FFPE samples of TNBC patients with high or low GD3S expression ( n = 20 total) were used to compare immune infiltration patterns, showing distinct fluorescence intensities for PANK, Ki67, CD163, CD86, CD56, CD8, CD4, FOXP3, and GD3S within tumor tissues. C H-scores for GD3S expression in BC patients categorized into high and low GD3S expression groups ( ****P < 0.0001). Immune cell counts using Visiopharm IHC image analysis software show differences in the expression of CD8, CD4, CD86, and CD56 between the high and low GD3S expression groups ( ****P < 0.0001; **P < 0.01). Scale bar in IHC images represents 55 µm with 20x magnification. Bar graphs display means ± standard deviations.

Article Snippet: We also purchased the following antibodies: pan-Cytokeratin (Proteintech, cat. #26411-1-AP, rabbit host) along with GD3S (Proteintech, cat. #24918-1-AP, rabbit host), CD45 (Fortislife, clone BL-178-1267, cat. #A700-012, rabbit host), Ki67 (Bethyl, clone BLR021E, cat. #A700-021CF, rabbit host), CD3 (Fortislife, clone BL-298-5012, cat. #A700-016F, rabbit host), CD8 (Fortislife, clone c8/144B, cat. #A500-012A, mouse host), CD4 (Bethyl, clone BL-155-1C11, cat. #A700-015, rabbit host), FOXP3 (Bethyl, clone BLR034F, cat. #A700-034, rabbit host), CD68 (Fortislife, cat. #A500-018ACF, mouse host), CD163 (Invitrogen, clone I0D6, cat. #MA5-11458, mouse host), CD86 (CST, clone E2G8P, cat. #76755SF, rabbit host), CD56 (Fortislife, clone BLR152J, cat. #A700-152, rabbit host) and human IgG1 Kappa antibody (hIgG, cat. #0151K-14, SouthernBiotech).

Techniques: Expressing, Multiplex Assay, Immunofluorescence, Fluorescence, Software

Journal: Computational and Mathematical Methods in Medicine

Article Title: miR-641 Inhibited Cell Proliferation and Induced Apoptosis by Targeting NUCKS1/PI3K/AKT Signaling Pathway in Breast Cancer

doi: 10.1155/2022/5203839

Figure Lengend Snippet: miR-641 inhibits the proliferation and promotes apoptosis of breast cancer cells. (a) Detection of transfection efficiency of miR-641 mimics in MDA-MB-231 and MCF7 cells; miR-641 expression was detected by real-time PCR; (b) Green fluorescent in MDA-MB-231 and MCF7 cells under a fluorescence microscope; (c–e) the proliferation abilities of transfected cells were detected by CCK-8 assay, EdU incorporation assay, and colony formation assay; (f) the protein levels of PCNA and Ki67 were measured by western blot; (g) apoptosis detection was performed using flow cytometry; (h) the protein levels of Bax, Bcl-2, caspase-3, and caspase-9 were measured by western blot. ∗∗ P < 0.01 vs. NC mimics.

Article Snippet: The antibodies used in this analysis included rabbit polyclonal to NUCKS1 (ab7770), proliferating cell nuclear antigen (PCNA; ab15212), Ki67 (ab15580), Bax (ab53154), Bcl-2 (ab196495), caspase-3 (ab13847), caspase-9 (ab52298), Cyclooxygenase-2 (Cox-2; ab15191), matrix metallopeptidase-2 (MMP-2; ab97779), MMP-9 (ab38898), phosphatidylinositol-3-kinases (PI3K; ab70912), p-PI3K (ab138364), protein-serine-threonine kinase (AKT; ab8805), and p-AKT (ab38449), purchased from Abcam (Cambridge, UK).

Techniques: Transfection, Expressing, Real-time Polymerase Chain Reaction, Fluorescence, Microscopy, CCK-8 Assay, Colony Assay, Western Blot, Flow Cytometry